Orientation Control of Photo-Immobilized Antibodies on the Surface of Azobenzene-Containing Polymers by the Introduction of Functional Groups 0comments
guest IP:96.0.219.* published in 2010-02-27 08:36:13
In our photo-induced immobilization technology for an antibody (IgG) using azopolymers the preface of COOH and NMe2 into the azopolymers which can introduce surface charges strongly affected the ...
In our photo-induced immobilization technology for an antibody (IgG) using azopolymers the preface of COOH and NMe2 into the azopolymers which can introduce surface charges strongly affected the immobilization properties such like the efficiency of immobilization and the activity of the immobilized IgG (i.e. the orientation of the immobilized IgG). The introduction of COOH promoted a more energetic orientation of the immobilized IgG. The orientation was determined during the adsorption process onto the azopolymer surface in solution before photo-immobilization and was maintained during the photo-immobilization. The surface charge of the azopolymer appears to be an important factor for IgG orientation which involves electrostatic interactions between its Fab and the azopolymer surface. Say what I think